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. 2018 Jan 6;19(1):167. doi: 10.3390/ijms19010167

Figure 2.

Figure 2

New MO sequences upregulate the SMN protein in HeLa cells. (A) A representative image of western blot analysis and densitometry analysis on HeLa cells treated with different MO sequences shows that the SMN protein levels increased after nucleofection with MO B and MO D. The combined treatment appeared to more efficiently increase SMN levels (*** p < 0.001) and was closer to the efficiency of MO-10-34 treatment compared to the other sequence alone (MO-10-34 vs. MO B + D p < 0.05; MO-10-34 vs. MO D p < 0.01; MO-10-34 vs. MO A, C and B p < 0.001); (B) Representative images of treated HeLa cells are shown. Nuclear gems (green) were detectable in all MO-treated cells compared to cells treated with scr-MO treated. In the right panel a magnification of the white frame of MO B + D image. Nuclei are labeled with diamidino-2-phenylindole dihydrochloride (DAPI) in blue; (C) Quantification results of the number of gems in HeLa cells treated with different MO sequences are shown. The greatest increase was observed after co-treatment with MO B + MO D (*** p < 0.001). Two independent observers examined at least 100 nuclei in randomly selected fields from each slide, and recoded the gems number. The values show the means + SD from 3 independent experiments. Scr-MO: scrambled-MO (white bar). Scale bar: 75 µm. MO B + D: 85 µm, insert 50 µm.