Figure 3.

Effect of the valproate (VPA) and resveratrol combination on RelA acetylation state in OGD-exposed primary cortical neurons. Co-immunoprecipitation (co-IP) analysis of RelA acetylation in nuclear extracts of OGD-exposed cells treated during the following 2 h with vehicle or drugs. The combination of the drugs (VPA 1 nmol/mL and resveratrol 3 nmol/mL) site-specifically decreased the RelA K310 acetylation. In the densitometry analysis of immunoblot bands relative to RelA, data were expressed as percentage of the corresponding control value. Bars depicted the mean ± s.e.m. of three separate experiments, * p < 0.05 and ** p < 0.01 versus the corresponding OGD value.