Figure 1.

Characterization of human immortalized myoblast cell lines. (A) Wild Type (W1 & W2) and DMD (D1, D2, D3 & D4) cell lines were seeded on Matrigel-coated 35 mm dishes in proliferative medium. The day after, proliferative medium was replaced or not (Undiff) by differentiation medium. Cells were differentiated over eight days (D8), then myosin heavy chain (MyHC) immunostaining and Hoechst nucleus staining were performed (scale bar: 50 µm); (B,C) Total proteins of wild type and DMD cell lines were extracted and 10 μg were separated by SDS-PAGE. Analysis of MyHC (B) and dystrophin (C) expression was performed by immunoblotting thanks to the use of specific antibodies. Actin is used as a loading control. Results are representative of three independent experiments.