Figure 2.

OGT activity inhibition by STO45849 (STO), an OGT inhibitor, rescued BMP2-induced ALP activity and osteogenic marker gene expression under excessive O-GlcNAcylation-inducing conditions. C2C12 cells were incubated for 48 h in the presence or absence of the indicated reagents, and osteogenic differentiation was examined by ALP staining (A); ALP activity assays (B); and qRT-PCR analysis of osteoblast differentiation marker genes (C). * p < 0.05 compared with the non-treatment control; # p < 0.05 for the indicated pairs.