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. 2018 Jan 9;19(1):202. doi: 10.3390/ijms19010202

Figure 5.

Figure 5

Excessive O-GlcNAcylation inducers suppressed Runx2 transcriptional activity, which was restored by OGT inhibitor (STO) addition or OGA overexpression. (A) C2C12 cells were transiently transfected with Myc-Runx2 expression plasmids and incubated under the indicated conditions for 24 h. Subsequently, immunoprecipitation with Runx2 antibody was performed, followed by immunoblotting with O-GlcNAc or Myc antibodies. High glucose (GC), glucosamine (GS), and N-acetylglucosamine (GN) enhanced Runx2 O-GlcNAcylation; (B–D) C2C12 cells were transiently transfected with the indicated expression plasmids and OSE-luc (a Runx2 reporter plasmid) and incubated for 24 h in the presence or absence of O-GlcNAcylation inducers and STO. Luciferase activity was then measured. * p < 0.05 compared with the non-treatment control; # p < 0.05 for the indicated pairs.