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. 2018 Jan 9;19(1):208. doi: 10.3390/ijms19010208

Figure 4.

Figure 4

Multiple effects of Aeginetia indica decoction on the HCV life cycle. (a) HCV RNA levels in Huh7.5.1 cells pretreated with vehicle or Aeginetia indica decoction (8 mg/mL) followed by HCV infection. The left panel rescales datum points in the boxed area of the right panel. Values are mean ± SEM summarized from three independent experiments and normalized against the values under the vehicle control (0 mg/mL). Asterisk indicates p < 0.05, t-test against the values under the vehicle control; (b) Representative image and summary of immunoblotting for HCV non-structural protein 3 (NS3) protein in Huh7.5.1 cells treated the same way as in (a). Samples from 72 h post HCV infection were analyzed. β-actin serves as a loading control; (c) Effects of Aeginetia indica decoction on HCV internal ribosome entry site (IRES)-mediated protein translation activity in Huh7.5.1 cells; (d) Effects of Aeginetia indica decoction on HCV RNA levels in HCV-infected Huh7.5.1 cells. Values were adjusted for loading (18S RNA) and standardized against the values under the vehicle control.