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. 2018 Jan 13;19(1):236. doi: 10.3390/ijms19010236

Figure 5.

Figure 5

CFL2 is a direct target gene of miR-3189-3p. (A) The putative miR-3189-3p binding sites in the CFL2 3′ untranslated region (3′-UTR) ,the sequences marked in red colour inside the box indicate seed sequences of miR-3189-3p which are conserved among different species. The underlined sequence ‘AAACCTT’ marked in red colour refers to the mutated bases in the sequence used to construct the mut-CFL2-3′-UTR-reporter vector. Luciferase activity in (B) HEK293T and (C) MGC803 cells after co-transfection of miR-3189-3p mimics or negative control with wt-CFL2-3′-UTR-reporter or mut-CFL2-3′-UTR-reporter constructs. (D) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and (E) Western blotting were used to detect CFL2 mRNA and protein expression in MGC803 cells after transfection with the miR-3189-3p mimics or negative control. Data were from three independent experiments and are presented as the mean ± SD. * p < 0.05.