Skip to main content
. 2018 Jan 13;19(1):237. doi: 10.3390/ijms19010237

Figure 4.

Figure 4

Age-related changes of relevant protein expression in untreated (A,B) and Lidocaine-treated (C,D) DBA/2J mice. (A) Western Blot images of Brain Derived Neurotrophic Factor (BDNF) (28 kDa), Tyrosine Receptor Kinase B (TrkB) (140 kDa), PSD95 (95 kDa), Synaptophysin (SYN) (38 kDa), Glial Fibrillary Acidic Protein (GFAP) (50 kDa) and Glyceraldehyde 3-phosphate Dehydrogenase GAPDH (37 kDa) proteins assessed in untreated mice of different ages (3 mice for age group, pooled retinas of both eyes); (B) Average protein expression (normalized protein/GAPDH ratio) as as function of age in untreated mice. Bars represent the average of 6 retinas for age group; (C) Representative Western Blot images of relevant retinal proteins in mice 6 and 8–10 old that received 4 retrobulbar injections of Lidocaine in the left eye and PBS in the right eye at 4 months of age; (D) Average protein expression in Lidocaine-treated (OS) and PBS-treated (OD) eyes. Bars represent the average ratios of 9 D2 mice of 6–10 months of age.