Quantitative RT-PCR validation. (A) Quantitative RT-PCR validated DEGs. DEGs (N1ICD, G2/mitotic-specific cyclin-B1 (CCNB1), Jagged 1 (JAG1), HEYL, HEY1, TIMP metallopeptidase inhibitor 1 (TIMP1), VGF nerve growth factor inducible (VGF), HES4, clusterin (CLU), transferrin receptor (TFRC), decorin (DCN), aldehyde dehydrogenase 1 family member A1 (ALDH1A1), actin, alpha, cardiac muscle 1 (ACTC1), secreted phosphoprotein 1 (SPP1), fibroblast growth factor 2 (FGF2) and C-C motif chemokine ligand 5 (CCL5)) belong to P-N1ICD vs. P-Control, and DEGs (actin, gamma 2, smooth muscle, enteric (ACTG2), calponin 1 (CNN1), heat shock protein beta-7 (HSPB7) and EGR1) belong to D-N1ICD vs. D-Control. (B) DEGs in gene expression profile. (C) Quantitative RT-PCR validated DEMs. DEMs (ssc-miR-221-3p, ssc-miR-369, ssc-miR-10-5p, ssc-miR-206, ssc-miR-935, ssc-miR-145-5p, ssc-miR-125a and ssc-miR-192) belong to P-N1ICD vs. P-Control, and DEMs (ssc-miR-331-5p, ssc-miR-139-5p and ssc-miR-574) belong to D-N1ICD vs. D-Control. (D) DEMs in the gene expression profile.