Separation of F3 fraction and the anticancer and antioxidant activities of the sub-fractions, S1, S2, and S3. (a) F3 fraction (Figure 1b and Figure 2), which was found exhibit high anticancer activity, was further separated by the LH-20 column, and three sub-fractions, S1 (1–39), S2 (40–96), and S3 (97–195), were obtained. (b) Anticancer activities of S1, S2, and S3 were determined as described in Figure 2a–c. Contents of total phenolics (c), flavonoids (d), and the reducing powers (e) and IC50 values for DPPH radical-scavenging activities (f) were detected and expressed as described in Figure 2. The statistic details of the data are as described in Figure 2.