Figure 4.

Separation and identification of the compounds in S3 fraction. S3 fraction (Figure 3) was further separated by Sephadex LH-20 chromatography and A280 peaks were monitored and combined as P1, P2, P3, and P4 (a). (b–f) Identification of the compounds in P1–P4 by HPLC analysis. (b) HPLC separation of the standards of catechin-type monomeric compounds. HPLC analysis of P1, 2, 3, and 4 (described in (a)) is shown in (c–f), respectively. Only EC (Epicatechin) was identified in P1 based on the comparison with the standards (b). Unknown compounds were also detected in P2 (d), P3 (e), and P4 (f). (g) Comparison of IC50 values for DPPH radical-scavenging activities of the reference Vit C, Litchi anthocyanin (cyanidin-3-rutinside) that was purified by LH-20 column, and the fractions P1–P4, expressed as micrograms gallic acid equivalents per milliliter (μg/mL) of the fractions. (h) Anticancer activities of the fractions P1–P4 at 5–30 μg/mL were determined as described in Figure 2, with 0.5 μg/mL cis-Dichlorodiamineplatinum (DDP) as the reference. The activity of the combination of an equal amount of P1–P4 was also detected. The statistic details of the data are as described in Figure 2.