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. 2018 Jan 21;19(1):312. doi: 10.3390/ijms19010312

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Aloperine induced caspase-dependent apoptosis in human thyroid cancer cells. (A) IHH-4 and (B) KMH-2 cells were incubated with aloperine, and the activation of caspase-8, -9 and -3 and PARP was detected by Western blot. (C) IHH-4 and (D) KMH-2 cells were pre-incubated with the pan-caspase inhibitor (Z-VAD-FMK) for 2 h and/or incubated with aloperine to confirm the activation of caspase-dependent apoptosis. The cellular apoptosis (C,D) was determined by flow cytometry with PI/Annexin-V double staining (E,F). Three independent experiments were confirmed. DMSO was used as a negative control. ** p < 0.01, *** p < 0.001, ^## p < 0.01.

Aloperine induced caspase-dependent apoptosis in human thyroid cancer cells. (A) IHH-4 and (B) KMH-2 cells were incubated with aloperine, and the activation of caspase-8, -9 and -3 and PARP was detected by Western blot. (C) IHH-4 and (D) KMH-2 cells were pre-incubated with the pan-caspase inhibitor (Z-VAD-FMK) for 2 h and/or incubated with aloperine to confirm the activation of caspase-dependent apoptosis. The cellular apoptosis (C,D) was determined by flow cytometry with PI/Annexin-V double staining (E,F). Three independent experiments were confirmed. DMSO was used as a negative control. ** p < 0.01, *** p < 0.001, ^## p < 0.01.