Table 1. List of differentially expressed proteins in the HepG2 cells treated with YK51 compound.
| Spot ID | Protein name (Gene symbols) | Swiss-Prot accession | Log normalized volume ± SDa | Fold changeb | Student’s t-test | Distinct sum MS/MS scorec | % Seq/Matched peptidesd | MW (kDa)/pIe | |
|---|---|---|---|---|---|---|---|---|---|
| Uninfected, Untreated (n = 5) | Uninfected, Treated (n = 5) | ||||||||
| 1 | T-complex protein 1 subunit theta (CCT8) | P50990 | 6.12 ± 0.20 | 5.56 ± 0.59 | −2.2 | 0.027 | 51.28 | 8/4 | 60.4/5.5 |
| 2 | Sepiapterin reductase (SPR) | P35270 | 6.51 ± 0.14 | 6.20 ± 0.30 | −1.9 | 0.021 | 43.36 | 12/3 | 31.3/8.5 |
| 3 | Protein phosphatase 1, catalytic subunit, alphaisoform (PPP1CA) | P62136 | 6.66 ± 0.07 | 6.41 ± 0.22 | −1.7 | 0.014 | 21.05 | 5/2 | 45.8/7.2 |
| 4 | Deoxythymidylate kinase (DTYMK) | P23919 | 6.49 ± 0.08 | 6.33 ± 0.08 | −1.5 | 0.004 | 1114.62 | 24/7 | 28.6/6.7 |
| 5 | Platelet-activating factor acetylhydrolase IB subunit gamma (PAFAH1B3) | Q15102 | 6.43 ± 0.07 | 6.24 ± 0.10 | −1.5 | 0.002 | 38.53 | 11/3 | 25.7/6.3 |
| 6 | COP9 signalosome complex subunit 5 (COPS5) | Q92905 | 6.59 ± 0.04 | 6.40 ± 0.17 | −1.5 | 0.013 | 44.20 | 6/3 | 42.6/9.4 |
| 7 | Annexin IV (ANXA4) | P09525 | 6.42 ± 0.04 | 6.22 ± 0.18 | −1.5 | 0.013 | 29.90 | 9/3 | 36.1/5.8 |
| 8 | Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform (PPP2R1A) | P30153 | 6.98 ± 0.10 | 6.80 ± 0.21 | −1.5 | 0.048 | 267.22 | 32/19 | 65.3/5.0 |
Notes.
a
Logarithm of normalized spot volume with standard deviation.
b
Negative sign (−) of fold change signifies decreased abundance of identified proteins compared to HepG2 cells without YK51 treatment.
c
Sum of the z-scores from each distinct detected peptide in a same protein species.
d
Percentage of protein sequence coverage (% Seq) and number of distinct detected peptides.
e
Molecular weight of protein in kilo Dalton and isoelectric focusing point of a protein.