Table 2. List of differentially expressed proteins in the DENV2-infected HepG2 cell with YK51 treatment.

SSpot #	Protein name (Gene symbols)	Swiss-Prot accession	Log normalized volume ± SDa		Fold changeb	Student’s t-test	Distinct sum MS/MS scorec	% Seq/ Matched peptidesd	MW (kDa)/pIe
			Infected, Untreated (n = 5)	Infected, Treated (n = 5)
1	Retinal dehydrogenase 1 (AL1A1)	P00352	6.64 ± 0.26	6.32 ± 0.20	−2.1	0.02	58.92	9/5	54.9/6.3
2	Retinal dehydrogenase 1 (AL1A1)	P00352	6.98 ± 0.18	6.63 ± 0.29	−2.1	0.01	328.30	39/20	54.9/6.3
3	Interleukin enhancer-binding factor 2 (ILF2)	Q12905	7.15 ± 0.18	6.79 ± 0.37	−2.0	0.03	134.79	27/10	44.7/5.2
4	Eukaryotic initiation factor 4A-I (eIF4A-1)	P60842	6.57 ± 0.16	6.25 ± 0.24	−2.0	0.01	291.69	45/19	46.2/5.3
5	Proliferation-associated protein 2G4 (PA2G4)	Q9UQ80	6.60 ± 0.19	6.26 ± 0.31	−2.0	0.02	77.32	13/4	43.8/6.1
6	Inosinicase (PUR9)	P31939	6.73 ± 0.13	6.43 ± 0.19	−2.0	0.01	359.25	28/24	64.6/6.27
7	Alanine aminotransferase 2 (ALAT2)	Q8TD30	6.97 ± 0.15	6.66 ± 0.25	−1.9	0.01	143.27	19/11	57.9/7.9
8	ATP synthase subunit beta (ATPB)	P06576	6.53 ± 0.14	6.29 ± 0.11	−1.8	0.00	23.53	6/2	56.9/5.4
9	leucineaminopeptidase 3 (AMPL)	P28838	6.54 ± 0.16	6.25 ± 0.32	−1.8	0.04	138.69	26/11	56.2/8.0
10	26S protease regulatory subunit 8 (PRS8)	P62195	6.78 ± 0.22	6.53 ± 0.17	−1.8	0.03	55.22	13/5	45.6/7.11
11	Glucose-6-phosphate 1-dehydrogenase (G6PDH)	P11413	6.91 ± 0.14	6.62 ± 0.25	−1.8	0.02	141.97	18/11	62.5/8.23
12	Protein disulfide-isomerase A6 (PDIA6)	Q15084	7.48 ± 0.17	7.21 ± 0.17	−1.8	0.01	281.29	33/18	53.9/5.17
13	Elongation factor 2 (EF-2)	P13639	6.54 ± 0.01	6.28 ± 0.21	−1.8	0.01	227	19/17	95.3/6.41
14	Protein disulfide-isomerase A3 (PDIA3)	P30101	7.32 ± 0.15	7.10 ± 0.20	−1.7	0.03	314.27	40/21	56.8/5.99
15	ATP synthase subunit beta (ATPB)	P06576	7.26 ± 0.17	7.04 ± 0.18	−1.7	0.03	270.75	41/15	56.6/5.26
16	Tubulin alpha-1B chain (TBA1B)	P68363	7.28 ± 0.17	7.03 ± 0.27	−1.7	0.04	415.15	55/23	50.2/4.94
17	T-complex protein 1 subunit alpha (TCP1A)	P17987	6.59 ± 0.01	6.33 ± 0.21	−1.7	0.01	66.01	8/4	60.3/5.8
18	Elongation factor Tu(EF-Tu)	P49411	7.06 ± 0.17	6.82 ± 0.24	−1.7	0.04	356.83	48/21	49.9/7.26
19	Protein disulfide-isomerase A6 (PDIA6)	Q15084	6.83 ± 0.12	6.62 ± 0.01	−1.7	0.00	28.31	7/2	48.1/4.95
20	Glutamate dehydrogenase 1 (GDH 1)	P00367	7.24 ± 0.14	7.01 ± 0.17	−1.5	0.05	200.33	31/12	61.4/7.66
21	Annexin A5 (ANXA5)	P08758	7.22 ± 0.01	7.03 ± 0.17	−1.5	0.02	224.97	39/14	35.9/4.94
22	Heat shock 70 kDa protein 9 (HSPA9)	P38646	7.23 ± 0.01	7.04 ± 0.20	−1.5	0.03	197.84	24/21	73.9/6.04
23	26S proteasome non-ATPase regulatory subunit 7 (PSMD7)	P51665	6.87 ± 0.01	6.70 ± 0.01	−1.5	0.00	59.57	19/5	37.0/6.29
24	Transaldolase, 1 (TALDO1)	P37837	7.09 ± 0.10	6.90 ± 0.19	−1.5	0.03	25.11	5/2	37.5/6.36
25	Proteasome activator complex subunit 1 (PSME1)	Q06323	7.30 ± 0.15	7.12 ± 0.12	−1.5	0.02	20.02	9/2	28.7/5.78
26	ATP-citrate synthase, isoform X1 (ACL)	P53396	6.50 ± 0.12	6.33 ± 0.13	−1.5	0.02	133.61	8/9	12.6/8.40
27	biliverdin IX alpha reductase (BVR A)	P53004	7.36 ± 0.01	7.19 ± 0.12	−1.5	0.01	39.14	9/3	33.5/5.91
28	Electron-transfer-flavoprotein, beta polypeptide (EFTB)	P38117	7.30 ± 0.13	7.13 ± 0.10	−1.5	0.01	50.81	14/4	27.9/8.55
29	Actin-related protein 2/3 complex subunit 4 (p20-ARC)	P59998	7.12 ± 0.12	6.94 ± 0.12	−1.5	0.01	49.75	22/4	19.7/8.54
30	Mitogen-activated protein kinase 1 (MAPK 1)	P28482	7.07 ± 0.12	6.87 ± 0.19	−1.5	0.03	62.04	16/4	41.4/6.5

Notes.

^aLogarithm of normalized spot volume with standard deviation.

^bNegative sign (−) of fold change signifies decreased abundance of identified proteins compared to DENV2-infected HepG2 without YK51 treatment.

^cSum of the z-scores from each distinct detected peptide in a same protein species.

^dPercentage of protein sequence coverage (% Seq) and number of distinct detected peptides.

^eMolecular weight of protein in kilo Dalton and isoelectric focusing point of a protein.