Table 1. Effect of the uptake of exogenous ldhD-siRNA by C1A germinating spores on the transcriptional level of ldhD relative to the housekeeping gene gapdh.
| Treatment | Final siRNA conc. (nM) | Copies of ldhD relative to gapdh1,2 | Fold change in transcription level (ΔΔCt) compared to untreated samples | Number of biological replicates | Fungal biomass yield (mg) at the time of sacrificing1 |
|---|---|---|---|---|---|
| ldhD-siRNA | 20 | 4.2E−03 ± 3E−03∗∗∗,a | 0.02 | 4 | 12.3 ± 5 |
| 50 | 4.4E−03 ± 2E−03∗∗∗,b | 0.02 | 5 | 9.3 ± 5.2 | |
| 75 | 3.6E−04 ± 1.8E−04∗∗∗,ab | 0.0017 | 4 | 15.4 ± 3.7 | |
| 100 | 6.1E−05 ± 2.4E−05∗∗∗,ab | 0.0003 | 4 | 15.9 ± 6 | |
| Untreated | NA | 0.21 ± 0.04 | 1 | 5 | 9.6 ± 2 |
| Unrelated-siRNA | 50 | 0.26 ± 0.07NS | 1.29 | 2 | 13.5 ± 3.8 |
Notes.
Values are average ± standard deviation. Student t-test was used to test the significance of the difference of the siRNA-treated samples averages from that of the untreated sample. All ldhD-siRNA treated samples showed a significant decrease in the transcriptional level of ldhD relative to the housekeeping gene gapdh compared to the untreated control. P-value of the significant difference; ***, p-value <0.00002; **, p-value = 0.0012; NS, not significant.
a,b When comparing the transcriptional level of ldhD relative to the housekeeping gene gapdh in samples treated with different concentration of ldhD-siRNA, a significant difference was observed with concentrations higher than 50 nM (p-value <0.05). Samples with the same letter were significantly different.