Fig. 4.

miR-302a-5p/367-3p inhibits malignant biological behaviour of Ishikawa and HEC-1A cell lines. a and b CCK-8 and EdU assays were used to evaluate effect of miR-302a-5p/367-3p on the proliferation Ishikawa and HEC-1A cell lines. c Representative images of the wound healing assay in Ishikawa and HEC-1A cell lines in response to changes in the expression of miR-302a-5p/367-3p. d Transwell assays were used to detect the effect of miR-302a-5p/367-3p expression on invasion in Ishikawa and HEC-1A cell lines. Representative images and accompanying statistical plots are presented. e Effects of miR-302a-5p/367-3p on apoptosis in Ishikawa and HEC-1A cell lines. f Effects of miR-302a-5p/367-3p on cell cycle distribution in Ishikawa and HEC-1A cell lines. Data are presented as the mean ± SEM (n = 3 per group). *P < 0.05, ** P < 0.01, *** P < 0.0001 vs. the miR-NC group