Fig. 5.

HMGA2 is a functional target of miR-302a-5p/367-3p in regulating EMT. a Western blot analysis of the expression of EMT-related proteins including E-cadherin, N-cadherin, MMP-2, MMP-9, Snail and Slug in response to HMGA2 in Ishikawa and HEC-1A cell lines. Relative expression of GAPDH as an endogenous control. Data are presented as the mean ± SEM, (n = 3 per group). b Western blot analysis of the expression of EMT-related protein including E-cadherin, N-cadherin, MMP-2, MMP-9, Snail and Slug in response to miR-302a-5p/367-3p. Data are presented as the mean ± SEM. (n = 3 per group), *P < 0.05, # P < 0.01, ▲ P < 0.0001. c and d Concentrations of MMP-2 and MMP-9 proteins in the supernatant of Ishikawa and HEC-1A cell cultures were detected by ELISA *P < 0.05, ** P < 0.01, *** P < 0.0001, (n = 3 per group). e and f qRT-PCR and western blotting of HMGA2-regulated RUNX1 expression in Ishikawa and HEC-1A cell lines. Data are presented as the mean ± SEM. *P < 0.05, ** P < 0.01, *** P < 0.0001 vs. the NC group, (n = 3 per group). g Western blot analysis of RUNX1 protein levels in Ishikawa and HEC-1A cell. Data are presented as the mean ± SEM. *P < 0.05, ** P < 0.01, *** P < 0.0001 vs. the NC group, (n = 3 per group)