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. 2017 Dec 18;2(2):155–172. doi: 10.1002/hep4.1131

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© 2017 The Authors. Hepatology Communications published by Wiley Periodicals, Inc., on behalf of the American Association for the Study of Liver Diseases.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Deglycosylation treatment converts 50‐kDa Msln to its 40‐KDa form. Results shown are from two separate experiments. Total protein lysates from TDE_CC and BDEneu cells in monolayer culture were treated with PNGaseF. Samples in Experiment 1 refer to those prepared from individual cultures. Nonenzyme‐treated samples omitted PNGaseF from the enzyme incubation step. Untreated refers to protein extraction samples prepared directly from the cultured cells without further processing. PNGaseF treatment was without effect on the BDEneu cells because these cells, unlike cultured TDE_CC cholangiocarcinoma cells, essentially expressed the 40‐kDa Msln form of Msln, with only marginal expression of the 50‐kDa form.