Figure 2.

Knockdown of USP15 inhibits the invasiveness of glioma cells, as determined by Transwell invasion assay. (A) USP15 depletion led to a significant reduction in the invasive ability of U87-MG cells (*P=0.002). (B) USP15 depletion led to a significant reduction in the invasive ability of U251-MG cells (*P=0.012). Altered protein levels of endogenous E-cadherin and typical mesenchymal markers, N-cadherin and vimentin, in (C) U87-MG and (D) U251-MG were detected by western blot analysis. USP15, ubiquitin specific peptidase 15; NC, negative control; KD, knockdown.