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. 2018 Feb 1;16:5. doi: 10.1186/s12964-018-0216-3

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — a Western blots analysis of the MKL1 protein expression at 48 h after transfected with siMKL1 (siMKL1-I, siMKL1-II and siMKL1-III) or siNC. GAPDH was used as an internal control. b The effect of knockdown HOTAIR or/and MKL1 on cell migration was determined by wound healing assay. c Quantification of the wound healing assay. d, e, f The effect of HOTAIR or/and MKL1 inhibition on cell invasion was determined in a Boyden chamber assay. And the number of cells on the underside of the filter was determined and significantly (P < 0.05) changed invasion is indicated. Data are presented as means ± S.D. and represent results from three independent experiments. Statistically significant differences are indicated: *, P < 0.05; **, P < 0.01 (Student’s t test)