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. 2018 Jan 10;15(3):3838–3845. doi: 10.3892/ol.2018.7759

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Figure 1. — RHEB expression in liver cancer cell lines and tissues. (A) RHEB was overexpressed in Hep3B, SMMC-7721, HepG2 and MHCC-97-H cells, but not in MHCC-97-L cells, as demonstrated by western blot analysis. (B) RHEB expression in liver cancer relative to the control gene GAPDH (5.00±0.34-fold) was upregulated compared with that in adjacent tissues (2.00±0.27-fold) and normal tissues (2.00±0.19-fold), as evaluated by gene microarray analysis. (C) RHEB messenger RNA level in liver cancer samples was upregulated compared with that in non-cancerous liver samples, as demonstrated by reverse transcription-quantitative polymerase chain reaction analysis. (D) RHEB was overexpressed in liver cancer tissues, but little or no expression was detected in normal liver tissues, as analyzed by immunohistochemistry. Magnification, ×200. RHEB, Ras homolog enriched in brain; mRNA, messenger RNA.