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. 2018 Jan 10;15(3):3838–3845. doi: 10.3892/ol.2018.7759

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Figure 4. — Influence of silencing RHEB on the biological behavior of the liver cancer cell line SMMC-7721. (A) Cell cycle changes of SMMC-7721 cells with low expression of RHEB were analyzed by flow cytometry. (B) Comparison of the cell cycle changes in SMMC-7721 cells treated with different shRNAs. (C) Analysis of soft agar colony-formation ability of SMMC-7721 cells with downregulated RHEB expression. The experiments were performed in triplicate, and representative images are shown. RHEB, Ras homolog enriched in brain; scr, scramble; FL2-A, fluorescence 2-area; shRNA, small hairpin RNA.