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. Author manuscript; available in PMC: 2018 Feb 2.
Published in final edited form as: Exp Eye Res. 2016 Dec 15;155:64–74. doi: 10.1016/j.exer.2016.12.006

Figure 3. Expression of CD5L/AIM in the retinal pigment epithelium.

Figure 3

Panel A, confocal microscopy on immortalized human ARPE-19 cells cultured in serum-free medium (SFM). CD5L/AIM immunofluorescence is shown in green (blue, DAPI nuclear stain). Note the diffuse granular cytoplasmic reactivity seen in the ARPE-19 cells and the more discrete, clump-like nuclear localization of the reactivity (see also magnified view in the inset in the top right hand corner, corresponding to the cropped area in Panel A). Panel B, negative control for ARPE-19 cells cultured in SFM labeled only with secondary antibody and DAPI for blue nuclear stain. Bar in Panels A and B = 50μm. Panel C, results of end-point PCR demonstrate expression of the expected CD5L/AIM transcript in ARPE-19 cells, which was further confirmed by sequencing of the band (GAPDH, positive control; empty lane, negative control). Panel D, results of Western blots performed on cell culture lysates of a primary human RPE cell line (hRPE) and an immortalized ARPE-19 cell line (green asterisk: CD5L/AIM band), confirming expression of CD5L/AIM also in both cell lines (red asterisk: RPE65 control band; last lane: human recombinant CD5L/AIM, positive control).