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. Author manuscript; available in PMC: 2018 Feb 2.
Published in final edited form as: Exp Eye Res. 2016 Dec 15;155:64–74. doi: 10.1016/j.exer.2016.12.006

Figure 4. Expression of CD5L/AIM in retinal microglial cells.

Figure 4

Immunostaining of a healthy human donor macular section with CD5L/AIM-specific Ab (green, Panel A) shows discrete staining in the inner half of the retina and very strong RPE staining, confirming the findings from Fig. 3. Note that a portion of the RPE signal was due to age-related autofluorescence and seen (in lesser amount) also in control experiments (not shown). The experiments summarized in Fig. 3 were conducted in part to overcome this issue. Subsequent confocal, higher magnification microscopy experiments were conducted on macular neuroretinal sections only (no RPE) also for this reason. Co-staining of a healthy human donor macular neuroretinal section with the same CD5L/AIM-specific Ab (green, Panel B) or the microglial specific marker Iba1 (red, Panel C) and a fluorescence-conjugated secondary Ab shows that CD5L/AIM colocalizes with Iba1 (merge, Panel D) in the same discrete fashion at the GCL, IPL and ONL level as seen in Panel A. DAPI (blue) was used as a nuclear stain. Abbreviations, nRet: neuroretina; PIS/POS: photoreceptor inner segment/outer segment; ONL: outer nuclear layer; OPL: outer plexiform layer; INL: inner nuclear layer; IPL: inner plexiform layer; GCL: ganglion cell layer. Bar in Panels A and B = 20μm.