Extended Data Fig. 6. Increasing neural activity in WT animals induces MIA behavioral phenotypes.

a, Representative images of c-Fos expression upon photostimulation of the S1DZ in WT offsprings from mothers injected with PBS at E12.5 (PBS offspring). In these animals, AAV₂-EF1α-DIO-EYFP, ChR2-EYFP, or NpHR-EYFP viruses were targeted to the S1DZ. Coronal sections of the brains were stained for c-Fos (red) and EYFP (green), and counterstained with neurotrace (NT, blue). Scale bar represents 100um. b–f, The marble burying index (the percentage of marbles buried during the 18-min marble burying test) (b), the % social target (the percentage of time spent investigating the social stimulus out of the total exploration time of both objects) (c), the total interaction time during the sociability test (d), the time spent in the center of an open field (e), and the total distance moved during the open field test (f) are plotted as averages from each individual 3-min sessions. Light blue indicates the laser ‘On’ sessions (‘Laser On-Off’: n=6, 5, and 8 mice/4-independent experiments; ‘Laser Off-On’: n=6, 7, and 10 mice/4-independent experiments from the AAV₂-hSyn-EYFP, ChR2-EYFP, or NpHR-EYFP injected PBS offspring). * p<0.05, ** p<0.01 as calculated by two-way repeated measures ANOVA with Tukey post-hoc tests. Graphs indicate mean ± s.e.m.