Extended Data Fig. 9. Unilateral increase in neural activity of the S1DZ region creates MIA behaviors in WT animals.

a, Schematic showing the unilateral virus injection and optic-fiber implantation in the S1DZ of WT animals injected with AAV₂-hSyn-EFYP, ChR2-EYFP, or NpHR-EFYP. The mouse brain in this figure has been reproduced with permission from Paxinos. b, Representative images of c-Fos expression upon photostimulation of the injection sites shown in (a). Coronal sections of the brains were stained for c-Fos (red) and EYFP (green), and counterstained with DAPI (blue). Scale bar represents 100μm. c, The percentage of EYFP⁺ neurons co-expressing c-Fos upon photostimulation of the injection site (n=9, 10, and 6 mice for WT animals injected with AAV₂-hSyn-EYFP, ChR2-EYFP, or NpHR-EYFP into S1DZ; 3-independent experiments). d, The number of c-Fos+ cells in the contralateral hemisphere of the injection site for the animals described in (c). e–h, The marble burying index (e), the % interaction (during the 1^st laser-on session) of the sociability test (f), the total interaction time (during the 1^st laser-on session) of the sociability test (g), the time spent in the center of an open field (during the 1^st laser-on session) (h), and the total distance moved during the open field test (during the 1^st laser-on session) (i) of animals prepared as in (a) (n=9, 12, and 10 for WT animals injected with AAV₂-hSyn-EYFP, ChR2-EYFP, or NpHR-EYFP into S1DZ; 3-independent experiments). * p<0.05, ** p<0.01 as calculated by two-way ANOVA with Tukey post-hoc tests (f) and one-way ANOVA with Tukey post-hoc tests (c,d,e,g,h,i). Graphs indicate mean ± s.e.m.