Fig. 6.

GGA3 levels are reduced in the hippocampus of 5XFAD mice with age. a Representative immunoblot of hippocampus homogenates from 2, 4, 7 and 12 months old GGA3WT;5XFAD, GGA3Het;5XFAD and GGA3KO;5XFAD mice probecr27d with anti-GGA3 (4167), anti-GGA1 (H-215), anti-GAPDH (MAB374) antibodies. b-c GGA3 and GGA1 levels, normalized to GAPDH in the hippocampus homogenates of GGA3WT;5XFAD and GGA3Het;5XFAD mice at 2, 4, 7 and 12 months of age, are expressed as relative values to the levels of GGA3 in 2 months old GGA3WT;5XFAD homogenates set to 1.0. Levels of GGA3 decrease in both GGA3WT;5XFAD and GGA3Het;5XFAD mice with age. In contrast, levels of GGA1 were decreased only in 12 months old GGA3WT;5XFAD mice compared to 2 months old GGA3WT;5XFAD mice. Total number of mice in each group is indicated within bars. All graphs represent mean ± SEM. Two-way ANOVA with Fisher’s LSD post hoc tests was applied to each genotype group. * p < 0.05, ** p < 0.01