Fig. 8.

Increased amyloid burden is associated with increased levels of APP in old female 5XFAD mice. a Representative immunoblot of hippocampus homogenates from 2 and 12 months old GGA3WT;5XFAD, GGA3Het;5XFAD, and GGA3KO;5XFAD male and female mice probed with anti-human APP (6E10) and anti-GAPDH (MAB374) antibodies. b Densitometry levels of transgenic human APP were quantified, normalized to GAPDH, and then expressed as relative values to their levels in 2 months old GGA3WT;5XFAD homogenates set to 1.0. For all GGA3 genotypes, 5XFAD females had significantly higher levels of APP compared to 5XFAD males at 12 months of age, but not 2 months of age (# p < 0.05, ## p < 0.01). GGA3KO;5XFAD males had reduced levels of APP compared to GGA3WT;5XFAD males (* p < 0.05). c The table shows summary of percentage increase of APP levels in 12 months old mice relative to genotype-matched 2 months old mice. ** p < 0.01, *** p < 0.001, **** p < 0.0001. Two-way ANOVA with Fisher’s LSD post hoc tests was applied to each sex or genotype group