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. Author manuscript; available in PMC: 2018 Feb 2.
Published in final edited form as: Chemistry. 2014 Sep 10;20(44):14410–14420. doi: 10.1002/chem.201403372

Figure 11.

Figure 11

Effect of complex 4 and 5 on mitochondrial membrane potential (MMP) and caspase activity in T98G glioma cells population at 24 h following treatment. (A) The relative number value of JC-1 red fluorescence to negative control was quantitated by flow cytometric analysis with JC-1 reagent for detection of MMP change in T98G cells. (B) Detection of apoptosis executioner caspase activity in T98G treated cells. Induction of apoptotic cell death can justify by measuring caspase-3/7 activity using the Promega Caspase-3/7Glo assay kit, and detection of luminescence is directly proportional to the level of caspase inside the cell.