Fig. 2.

Phylogenetic tree indicating current phylogenetic placement of strain JAB1.The phylogenetic tree was constructed in MEGA7 [62] using secondary structure-based alignment of 16S rRNA gene sequences of the JAB1 strain (four copies of the 16S rRNA gene retrieved from the JAB1 genome) and its closest relatives [63]. The evolutionary distances were computed using the Kimura 2-parameter method [64]. All positions with less than 95% site coverage were eliminated; there were a total of 1375 positions in the final dataset. The tree construction method used was Neighbor-Joining [65]. The bootstrap test (1000 replicates) was used to test tree topology [66]; only bootstrap values >70 are indicated