Fig. 1.

Determination of frequencies of Tregs and LAP⁺ macrophages and monocytes. a, b Gating of CD4⁺ T lymphocytes and determination of the proportions of three subpopulations in the peripheral blood (a) and peritoneal fluid (b): CD45RA⁺FoxP3^low resting Tregs (rTregs), CD45RA⁻FoxP3^high effector Tregs (eTregs), and CD45RA⁻FoxP3^low non-Tregs (non-Tregs). c, d Side light scattering and staining with CD11b gating to detect CD11b⁺ monocytes and macrophages from the peripheral blood (c) and peritoneal fluid (d). e Staining with isotype control, CD11b⁺LAP⁺ monocytes from the peripheral blood, and CD11b⁺LAP⁺ macrophages from the peritoneal fluid