Table 2. Two-way repeated-measures ANOVA results for abundance (N), richness (S), Shannon’s diversity index (H’), Simpson’s diversity index (D) and Pielou’s evenness index (J) of non-target arthropods collected by Moericke traps (yellow pan) in non-Bt (with and without insecticides) and Bt (DAS-81419-2) soybean fields at three sites over two to three years in Brazil.

Site	Year	Diversity indices	Soybean treatments			Two-way ANOVA
			Non-sprayed non-Bt	Sprayed non-Bt	DAS-81419-2	Soybean treat. (A)		Sampling time (B)		Interaction (A x B)
						F	P	F	P	F	P
Castro	2012	N	1161.50 ± 122.52 b	966.25 ± 126.53 c	1418.25 ± 165.52 a	39.78	0.002	155.06	< 0.001	8.58	< 0.001
		S	28.08 ± 1.47	26.83 ± 1.27	32.42 ± 1.40	5.29	0.075	5.93	0.032	0.83	0.572
		H’	1.86 ± 0.05	1.89 ± 0.08	1.83 ± 0.10	0.76	0.525	481.13	< 0.001	7.49	0.002
		D	0.77 ± 0.01	0.78 ± 0.02	0.74 ± 0.02	2.66	0.184	129.84	< 0.001	8.35	0.001
		J	0.24 ± 0.02 ab	0.26 ± 0.02 a	0.20 ± 0.02 b	13.76	0.016	36.89	< 0.001	3.56	0.029
	2013	N	220.60 ± 25.07	208.60 ± 15.73	204.00 ± 22.47	0.61	0.588	6.38	0.013	0.23	0.980
		S	29.40 ± 1.77 ab	31.33 ± 1.78 a	25.93 ± 1.50 b	8.85	0.034	14.14	0.001	0.97	0.491
		H’	2.40 ± 0.05	2.49 ± 0.10	2.31 ± 0.06	4.34	0.099	7.91	0.007	2.03	0.109
		D	0.83 ± 0.01	0.84 ± 0.02	0.83 ± 0.01	0.55	0.614	6.62	0.012	1.78	0.156
		J	0.41 ± 0.04	0.41 ± 0.02	0.42 ± 0.03	0.02	0.979	5.18	0.023	0.23	0.980
Montividiu	2011	N	330.42 ± 76.83	368.58 ± 77.27	324.92 ± 61.71	1.59	0.311	84.23	< 0.001	4.55	0.012
		S	24.17 ± 1.35	24.17 ± 1.29	25.25 ± 1.22	0.44	0.674	18.17	0.002	0.58	0.737
		H’	1.74 ± 0.17	1.62 ± 0.10	1.71 ± 0.11	2.07	0.242	21.57	0.001	2.59	0.076
		D	0.67 ± 0.05	0.64 ± 0.03	0.64 ± 0.03	0.77	0.523	10.91	0.008	2.73	0.066
		J	0.26 ± 0.03	0.22 ± 0.02	0.23 ± 0.02	1.65	0.299	17.85	0.002	3.20	0.041
	2012	N	596.50 ± 227.99	581.75 ± 219.17	896.17 ± 441.55	0.35	0.723	83.34	< 0.001	2.34	0.099
		S	24.67 ± 2.57	24.25 ± 3.04	20.25 ± 2.35	4.94	0.083	10.72	0.008	1.64	0.219
		H’	1.67 ± 0.13	1.68 ± 0.14	1.59 ± 0.17	1.98	0.252	64.04	< 0.001	0.73	0.637
		D	0.67 ± 0.04	0.69 ± 0.05	0.65 ± 0.05	1.28	0.372	40.45	< 0.001	1.19	0.372
		J	0.26 ± 0.05	0.28 ± 0.05	0.32 ± 0.06	1.75	0.285	70.43	< 0.001	1.95	0.153
	2013	N	395.93 ± 81.60	409.87 ± 85.60	460.87 ± 85.34	0.96	0.458	43.43	< 0.001	0.92	0.524
		S	28.80 ± 2.53	29.27 ± 2.60	31.73 ± 2.32	4.86	0.085	60.57	< 0.001	4.89	0.003
		H’	1.72 ± 0.10	1.63 ± 0.15	1.62 ± 0.13	0.65	0.570	16.04	< 0.001	1.50	0.233
		D	0.63 ± 0.04	0.58 ± 0.05	0.57 ± 0.04	1.29	0.370	17.20	< 0.001	1.75	0.161
		J	0.23 ± 0.04	0.21 ± 0.04	0.19 ± 0.04	0.98	0.448	43.77	< 0.001	1.62	0.197
Uberlândia	2011	N	353.00 ± 53.59	390.42 ± 69.60	376.00 ± 61.37	0.20	0.826	29.37	< 0.001	5.18	0.008
		S	39.17 ± 2.38	39.33 ± 2.87	45.08 ± 4.77	2.94	0.164	21.59	0.001	5.91	0.004
		H’	2.34 ± 0.06 ab	2.33 ± 0.13 b	2.51 ± 0.05 a	8.99	0.033	16.64	0.003	3.23	0.040
		D	0.80 ± 0.02	0.79 ± 0.02	0.83 ± 0.01	4.94	0.083	19.79	0.002	3.66	0.026
		J	0.29 ± 0.03	0.29 ± 0.04	0.31 ± 0.03	0.59	0.594	29.71	< 0.001	9.38	< 0.001
	2012	N	3918.50 ± 1045.77	3951.08 ± 858.45	3163.08 ± 950.32	0.99	0.446	39.60	< 0.001	5.76	0.005
		S	60.92 ± 3.50	54.42 ± 5.06	61.83 ± 4.42	0.77	0.520	68.16	< 0.001	2.36	0.096
		H’	1.33 ± 0.18	1.33 ± 0.13	1.62 ± 0.20	5.35	0.074	58.33	< 0.001	3.38	0.035
		D	0.50 ± 0.07	0.56 ± 0.05	0.58 ± 0.07	6.43	0.056	53.29	< 0.001	1.82	0.178
		J	0.08 ± 0.02	0.10 ± 0.03	0.10 ± 0.02	5.39	0.073	59.40	< 0.001	5.93	0.004

P-values highlighted in bold are statistically significant (α = 0.05).

Degrees of freedom: soybean treatments = 2; sampling time = 3 (2011 and 2012) and 4 (2013); interaction = 6 (2011 and 2012) and 8 (2013); residual = 12 (2011 and 2012) and 16 (2013).

Means (± SE) followed by different letters were significantly different within rows (Tukey’s test, α = 0.05).

Abundance was log(x + 1) transformed prior to analysis. Non-transformed means are presented.