Fig 4. CeCYLD can inhibit the activation of NF-kappaB.

HEK 293T cells (2x10⁵ cells per well in 12-well plates) were cotransfected with a NF-κB-dependent luciferase reporter plasmid (3XκBL), a β-galactosidase expression plasmid for transfection efficiency normalization (pGKβgal), and 0.4μg, 1.4μg and 0.4μg of plasmids expressing HsCYLD, CeCYLD and CeCYLDC774S respectively. After 18h, the cells were harvested and lysed in luciferase lysis buffer (Promega). The lysates were assayed for luciferase and β-galactosidase activities. (A) Values are shown as the mean +/- standard error of relative luciferase activity from six independent experiments. The values that were compared statistically are indicated by horizontal lines. (B) Representative immunoblot showing the expression levels of transfected proteins and β-actin. *p< 0.05, ***p<0.001.