Fig 5. CeCYLD can inhibit the activation of AP1.
HEK 293T cells (2x105 cells per well in 12-well plates) were cotransfected with a AP1-dependent luciferase reporter plasmid (7xAP1), a β-galactosidase expression plasmid for transfection efficiency normalization (pGKβgal), and 0.1μg, 1μg and 0.3μg of plasmids expressing HsCYLD, CeCYLD and CeCYLDC774S respectively. After 18h, the cells were harvested and lysed in luciferase lysis buffer (Promega). The lysates were assayed for luciferase and β-galactosidase activities. (A) Values are shown as the mean +/- standard error of relative luciferase activity from five independent experiments. The values that were compared statistically are indicated by horizontal lines. (B) Representative immunoblot showing the expression levels of transfected proteins and β-actin. *p< 0.05, **p<0.01.