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. 2018 Jan 23;14(1):e1007180. doi: 10.1371/journal.pgen.1007180

Fig 4. hb is expressed in migratory subperineurial glia cells.

Fig 4

(A-C) hb expression (VT038544-Gal4 driving histone-bound RFP (UAS-H2B::RFP) (A-C), red in A”-C”) co-localizes (white arrows) with the pan-glial marker Repo (detected with rabbit α-Repo antibody (A’-C’), green in A”-C”) in two large cells. These cells migrate from the optic stalk during L2 stage (A”) into the posterior end of the eye-antennal disc at mid L3 stage (B”) and are located in the posterior region of the retinal field, at each side of the optic stalk, at late L3 stage (C”). (VT038544-Gal4 driver line obtained from the Vienna Tile collection, see S4 Fig for details) (D) Hb (detected with rabbit α-Hb antibody, red in D”) is present in the same cells (white arrows) that express the subperineurial glia cell marker moody (D’) (moody-Gal4 driving UAS-GFP, green in D”). In all pictures, anterior is to the right. Eye disc (ed), optic stalk (os). Scale bar = 20 μm.