Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Reference or source |
|---|---|---|
| P. chlororaphis | ||
| 30-84WT | PCA+, 2-OH-PCA+, 2-OH-PHZ+, RifR, Wild type | Pierson and Thomashow (1992) |
| 30-84PCA | PCA+, phzO::Tn5, KmR | Maddula et al. (2008) |
| 30-84O* | PCA+, 2-OH-PCA+, 2-OH-PHZ+, RifR, 2OHPCA over producer, 30-84WT containing plasmid pKM-phzO* | Maddula et al. (2008) |
| 30-84ZN | Phz−, phzB::lacZ | Wood et al. (1997) |
| 30-84H | PCA+, PCN+, 30-84PCA containing plasmid pGT2-phzH | This study |
| 30-84S | PCA+, 1OHPZ+, 30-84PCA containing plasmid pGT2-phzS | This study |
| 30-84M | PCA+, 5MPCA+, 30-84PCA containing plasmid pGT2-phzM | This study |
| 30-84MS | PCA+, PYO+, 30-84PCA containing plasmid pGT2-phzMS | This study |
| ATCC17411 | PCA+, PCN+ | Mavrodi et al. (2001) |
| P. synxantha 2-79 | PCA+ | Weller (1983) |
| E. coli | ||
| DH5α | F-recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 Δ(argF-lacZYA)II69 ϕ80lacZ ΔM15 λ- | Gibco-ThermoFisher (Waltham, MA, USA) |
| HB101 | F- hsdS20(rB-mB-) supE44 recA1 ara14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-5 | Gibco-ThermoFisher |
| Plasmid | ||
| pCR2.1-TOPO | AmpR, KmR, T7 promoters, f1 ori, lacZα | Invitrogen Life Technologies |
| TOPO-phzH | pCR2.1-TOPO carrying a 1.9-kb DNA fragment containing phzH gene | This study |
| TOPO-phzS | pCR2.1-TOPO carrying a 1.2-kb DNA fragment containing phzS gene | This study |
| TOPO-phzM | pCR2.1-TOPO carrying a 1.2-kb DNA fragment containing phzM gene | This study |
| TOPO-phzM_BB | pCR2.1-TOPO carrying a 1.2-kb DNA fragment containing phzM gene | This study |
| pGT2 | pProbe-GT′: pVS1 replicon, p15a origin of replication, gfp transcriptional fusion; GmR | Miller et al. (2000) |
| pGT2-PPsP | pGT2 carrying a 127-bp DNA fragment containing truncated promoter sequence of phzX gene (PPsP) | This study |
| pKT2- PPsPlacZ | pKT2 carrying a 127-bp DNA fragment containing truncated promoter sequence of phzX gene (PPsP) fused to lacZ; KmR | Yu (2016) |
| pGT2-phzH | pGT2-PsP carrying a 1.9-kb DNA fragment containing phzH gene | This study |
| pGT2-phzS | pGT2-PsP carrying a 1.2-kb DNA fragment containing phzS gene | This study |
| pGT2-phzM | pGT2-PsP carrying a 1.2-kb DNA fragment containing phzM gene | This study |
| pGT2-phzMS | pGT2-PsP carrying a 2.4-kb DNA fragment containing phzM and phzS gene together | This study |
a
KmR, AmpR, GmR and RifR = kanamycin, ampicillin, gentamicin and rifampin resistance, respectively.