Table 1.
Primers used in RT-PCR analysis for virus detection
| Virus | Name | Sequences (5′ to 3′) | Base pairs | Product size (bp) | Target gene | Annealing temperature (°C) |
|---|---|---|---|---|---|---|
| CVX | CVX 678 UP | ATG TCT ACT ACT GGA GTC CA | 20 | 678 | CP | 58 |
| CVX 678 DN | CTA CTC AGG GCC TGG GAG AA | 20 | ||||
|
| ||||||
| CMMoV | CMMoV 486 UP | ATG GCG GGT TCT TAC ACC AA | 20 | 486 | CP | 57 |
| CMMoV 486 DN | CTA GGT GTG GCA CCT AAG GT | 20 | ||||
|
| ||||||
| OpVX | OpVX 690 UP | ATG GCT TCC ACT CCA CAG AC | 20 | 690 | CP | 57 |
| OpVX 690 DN | TTA TTC AGG ACC TGG TAG GA | 20 | ||||
|
| ||||||
| PiVX | PiVX 678 UP | ATG GCT ACT CAA ACA GCA CAA | 21 | 678 | CP | 57 |
| PiVX 678 DN | CTA CTC TGG GGA GGG AAG | 18 | ||||
|
| ||||||
| RCNaV | RCNaV 513 UP | ATG CCT TAC ATC AAC GTA CA | 20 | 513 | CP | 55 |
| RCNaV 513 DN | TTA AGT ACG ACT GTC ACT TG | 20 | ||||
|
| ||||||
| SchVX | SchVX 678 UP | ATG TCG ACC ACT CCA TCT TC | 20 | 678 | CP | 58 |
| SchVX 678 DN | TTA TTC AGG GGA TGG TAG TA | 20 | ||||
|
| ||||||
| ZyVX | ZyVX 681 UP | ATG TCT AAC ACT GCA GGA GT | 20 | 681 | CP | 58 |
| ZyVX 681 DN | TCA TTC GGG ACC CGG TAG GA | 20 | ||||