Fig. 4.

Symptom expression of Capsicum annuum S3669 mechanically inoculated with Tomato spotted wilt virus (TSWV)-Pap and subsequently maintained at 25 ± 2°C and 30 ± 2°C. A). A) Hypersensitivity reaction (HR) occurred in inoculated leaves of plants maintained at 25 ± 2°C at 7dpi without systemic infection; B) systemic infection of plants maintained at 30 ± 2°C at 10 dpi; C) recovery from infection at 15 dpi (arrow); D) RT-PCR with RNA extracted from 4 plants that exhibited systemic symptoms revealed TSWV-specific bands at 10 dpi, while TSWV-specific bands were not observed in 2 of 4 plants at 15 dpi.