Figure 6.
(a–d) Cell viability of 3D bioprinted hydrogel bioinks encapsulated with odontoblast-like cells (OD21s) at day 5 as determined by a live-dead assay kit. (e) Pure alginate hydrogels showed a consistent drop in cell viability over time, whereas hybrid alg-dent gels, especially at ratios of 1:1 and 1:2 had consistently higher cell viability. (f) Viability of human dental stem cells from the apical papilla (SCAPs) printed using a 1:1 alg-dent hybrid hydrogel bioink had viability consistently above 90% for at least 5 days. 1:1 alg-dent hybrid hydrogel bioink was used for all subsequent experiments. (2 mm scale bar)