Table 4.
Putative Homarus americanus neuropeptide-encoding transcripts identified via in silico transcriptome mining of a de novo assembled transcriptome for the eyestalk ganglia (lamina ganglionaris, medulla externa, medulla interna and medulla terminalis) and the proteins deduced from them
| Peptide family | Transcript/protein identifications | tblastn search statistics | |||||
|---|---|---|---|---|---|---|---|
| Transcript | Deduced protein | ||||||
| Identification No. | Length* | Name | Length† | Type | Score | E-value | |
| ACP | TR10790|c0_g1_i3 | 1033 | Prepro-ACP | 104 | F | 211 | 4e-55 |
| TR10790|c0_g1_i2 | 1077 | Prepro-ACP | 104 | F | 211 | 4e-55 | |
| TR10790|c0_g1_i1 | 1685 | Prepro-ACP | 104 | F | 211 | 4e-55 | |
| AST-A | TR19564|c0_g1_i1 | 1395 | Prepro-AST-A sv2 | 378 | N | 552 | 1e-156 |
| TR19564|c1_g1_i1 | 3284 | Prepro-AST-A sv2 | 189 | C | 357 | 7e-98 | |
| AST-B | TR68637|c1_g2_i2 | 647 | Prepro-AST-B | 157 | N | 120 | 1e-27 |
| TR68637|c1_g1_i2 | 950 | Prepro-AST-B sv1 | 153 | C | 69 | 3e-12 | |
| TR68637|c1_g1_i1 | 1058 | Prepro-AST-B sv2 | 189 | C | 69 | 3e-12 | |
| AST-C | TR39168|c1_g1_i1 | 1163 | Prepro-AST-C I | 65 | C | 97 | 1e-20 |
| TR48133|c0_g1_i1 | 1624 | Prepro-AST-C II | 105 | F | 174 | 4e-44 | |
| TR20160|c0_g1_i1 | 1609 | Prepro-AST-C III | 148 | F | 29 | 3.4 | |
| Allatotropin | |||||||
| Bursicon α | TR30087|c0_g1_i1 | 372 | Pre-bursicon α v2 | 100 | N | 209 | 2e-54 |
| Bursicon β | |||||||
| CAP2b | |||||||
| CCHamide | TR54993|c0_g1_i5 | 2717 | Prepro-CCHamide I | 116 | F | 237 | 6e-63 |
| TR54993|c0_g1_i4 | 3448 | Prepro-CCHamide I | 116 | F | 237 | 6e-63 | |
| TR54993|c0_g1_i3 | 3452 | Prepro-CCHamide I | 116 | F | 237 | 6e-63 | |
| TR54993|c0_g1_i2 | 3459 | Prepro-CCHamide I | 116 | F | 237 | 6e-63 | |
| TR54993|c0_g1_i1 | 3476 | Prepro-CCHamide I | 116 | F | 237 | 6e-63 | |
| TR75050|c0_g1_i1 | 3267 | Prepro-CCHamide II sv1 | 252 | F | 470 | 1e-132 | |
| TR75050|c0_g1_i3 | 3294 | Prepro-CCHamide II sv2 | 261 | F | 462 | 1e-130 | |
| TR75050|c0_g1_i2 | 1277 | Prepro-CCHamide II sv3 | 266 | F | 458 | 1e-129 | |
| TR75050|c0_g1_i4 | 1304 | Prepro-CCHamide II sv4 | 275 | F | 451 | 1e-126 | |
| Corazonin | TR17179|c1_g1_i6 | 1716 | Prepro-corazonin | 109 | F | 135 | 2e-32 |
| TR17179|c1_g1_i3 | 1716 | Prepro-corazonin | 109 | F | 135 | 2e-32 | |
| TR17179|c1_g1_i2 | 1715 | Prepro-corazonin | 109 | F | 135 | 2e-32 | |
| TR17179|c1_g1_i1 | 1715 | Prepro-corazonin | 109 | F | 135 | 2e-32 | |
| TR17179|c1_g1_i4 | 631 | Prepro-corazonin | 22 | C | 49 | 3e-06 | |
| CCAP | TR69144|c0_g1_i2 | 1098 | Prepro-CCAP | 140 | F | 254 | 5e-68 |
| TR69144|c0_g1_i1 | 1113 | Prepro-CCAP | 140 | F | 254 | 5e-68 | |
| CHH (CHH subgroup) | TR1098|c3_g6_i2 | 649 | Prepro-CHH Ia v1 | 134 | F | 260 | 6e-70 |
| TR1098|c3_g6_i3 | 497 | Prepro-CHH Ia v2 | 133 | F | 243 | 7e-65 | |
| TR1098|c3_g6_i4 | 589 | Prepro-CHH Ia v3 | 133 | F | 241 | 3e-64 | |
| TR1098|c3_g6_i1 | 747 | Prepro-CHH Ib v2 | 131 | F | 217 | 7e-57 | |
| TR25279|c0_g1_i1 | 673 | Prepro-CHH II sv2 | 111 | F | 87 | 1e-17 | |
| CHH (MIH subgroup) | TR12133|c0_g1_i1 | 1451 | Pre-MIH I v2 | 112 | F | 199 | 1e-51 |
| TR9706|c0_g1_i1 | 837 | Pre-MIH II | 111 | F | 87 | 1e-17 | |
| TR58586|c0_g1_i1 | 1736 | Pre-MIH III | 119 | F | 48 | 7e-06 | |
| DENamide | |||||||
| DH31 | TR60801|c0_g1_i2 | 2393 | Prepro-DH31 sv1 | 135 | F | 211 | 4e-55 |
| TR60801|c0_g1_i1 | 2371 | Prepro-DH31 sv2 | 128 | F | 190 | 1e-48 | |
| DH44 | TR48947|c0_g1_i2 | 1198 | Prepro-DH44 | 285 | F | 416 | 1e-116 |
| TR48947|c0_g1_i1 | 1551 | Prepro-DH44 | 285 | F | 416 | 1e-116 | |
| DXXRLamide | |||||||
| ETH | |||||||
| EH | TR8891|c0_g1_i1 | 555 | Pre-EH I | 88 | F | 120 | 1e-27 |
| TR33899|c2_g1_i1 | 2290 | Pre-EH II | 82 | F | 57 | 2e-08 | |
| Elevenin | TR73513|c0_g1_i1 | 884 | Prepro-elevenin | 127 | F | 39 | 0.003 |
| FLP | TR10371|c0_g1_i2 | 3112 | Prepro-FLP | 358 | F | 562 | 1e-160 |
| TR10371|c0_g1_i1 | 3101 | Prepro-FLP | 358 | F | 562 | 1e-160 | |
| FXGGXamide | |||||||
| GPα2 | TR7537|c0_g1_i1 | 2897 | Pre-GPα2 | 120 | F | 175 | 2e-44 |
| GPβ5 | TR31071|c0_g1_i2 | 2321 | Pre-GPβ5 | 163 | F | 170 | 8e-43 |
| TR31071|c0_g1_i1 | 2293 | Pre-GPβ5 | 163 | F | 170 | 8e-43 | |
| GSEFLamide | TR71156|c0_g1_i2 | 966 | Prepro-GSEFLamide sv1 | 268 | N | 39 | 0.003 |
| TR71156|c0_g1_i1 | 996 | Prepro-GSEFLamide sv2 | 278 | N | 39 | 0.003 | |
| TR33789|c3_g2_i1 | 1470 | Prepro-GSEFLamide | 35 | C | 75 | 3e-14 | |
| HIGSLYRamide | |||||||
| ILP | |||||||
| Intocin | TR17969|c0_g3_i1 | 1121 | Prepro-intocin | 154 | F | 309 | 2e-84 |
| TR17969|c0_g2_i1 | 1121 | Prepro-intocin | 154 | F | 309 | 2e-84 | |
| TR17969|c0_g1_i1 | 1121 | Prepro-intocin | 154 | F | 309 | 2e-84 | |
| Leucokinin | TR63890|c0_g1_i1 | 2739 | Prepro-leucokinin v1 | 606 | F | 920 | 0.0 |
| TR63890|c0_g1_i2 | 1230 | Prepro-leucokinin v2 | 130 | C | 64 | 1e-09 | |
| Myosuppressin | TR50807|c0_g1_i1 | 1600 | Prepro-myosuppressin | 100 | F | 169 | 2e-42 |
| Neuroparsin | TR33370|c0_g1_i1 | 726 | Pre-neuroparsin I | 98 | F | 207 | 5e-54 |
| TR49668|c0_g1_i1 | 1130 | Pre-neuroparsin II | 98 | F | 105 | 2e-23 | |
| TR70851|c4_g1_i1 | 1490 | Pre-neuroparsin III | 101 | F | 101 | 5e-22 | |
| TR43449|c0_g1_i2 | 1897 | Pre-neuroparsin IV | 103 | F | 100 | 2e-21 | |
| TR43449|c0_g1_i1 | 878 | Pre-neuroparsin IV | 103 | F | 100 | 2e-21 | |
| NPF | TR52169|c0_g1_i1 | 1470 | Prepro-NPF I sv2 | 132 | F | 161 | 4e-40 |
| TR47662|c0_g1_i1 | 1008 | Prepro-NPF II sv1 | 79 | F | 34 | 0.081 | |
| TR47662|c0_g1_i2 | 1119 | Prepro-NPF II sv2 | 116 | F | 28 | 4.4 | |
| NPLP1 | |||||||
| NPLP2 | |||||||
| NPLP3 | |||||||
| NPLP4 | |||||||
| Orcokinin | TR61279|c0_g2_i1 | 514 | Prepro-orcokinin II sv2 | 156 | N | 225 | 8e-59 |
| TR61279|c0_g1_i1 | 504 | Prepro-orcokinin II sv2 | 152 | N | 217 | 2e-56 | |
| TR4139|c0_g1_i1 | 1307 | Prepro-orcokinin I/II | 118 | C | 251 | 1e-66 | |
| TR4139|c0_g1_i2 | 1241 | Prepro-orcokinin IV | 96 | C | 203 | 3e-52 | |
| PDH | TR23970|c0_g1_i1 | 1058 | Prepro-PDH I | 79 | F | 122 | 2e-28 |
| TR9861|c0_g4_i1 | 481 | Prepro-PDH II | 79 | F | 101 | 4e-22 | |
| TR9861|c0_g3_i1 | 481 | Prepro-PDH II | 79 | F | 101 | 4e-22 | |
| TR9861|c0_g2_i1 | 481 | Prepro-PDH II | 79 | F | 101 | 4e-22 | |
| TR9861|c0_g1_i1 | 714 | Prepro-PDH II | 79 | F | 101 | 4e-22 | |
| Proctolin | TR11540|c0_g1_i2 | 962 | Prepro-proctolin | 88 | F | 111 | 4e-25 |
| TR11540|c0_g1_i1 | 951 | Prepro-proctolin | 88 | F | 111 | 4e-25 | |
| PTTH | |||||||
| Pyrokinin | TR21391|c0_g1_i1 | 1766 | Prepro-pyrokinin | 385 | F | 528 | 1e-150 |
| RPCH | TR11499|c0_g1_i1 | 770 | Prepro-RPCH | 98 | F | 101 | 4e-22 |
| RYamide | TR37059|c0_g1_i1 | 707 | Prepro-RYamide | 135 | F | 160 | 6e-40 |
| sNPF | TR46457|c1_g1_i2 | 677 | Prepro-sNPF sv1 | 96 | F | 137 | 9e-33 |
| TR46457|c1_g1_i1 | 663 | Prepro-sNPF sv1 | 96 | F | 137 | 9e-33 | |
| TR46457|c1_g1_i4 | 773 | Prepro-sNPF sv2 | 128 | F | 182 | 1e-46 | |
| TR46457|c1_g1_i3 | 759 | Prepro-sNPF sv2 | 128 | F | 182 | 1e-46 | |
| SIFamide | TR65061|c0_g1_i1 | 1734 | Prepro-SIFamide sv1 | 80 | F | 132 | 2e-31 |
| TR65061|c0_g1_i2 | 1302 | Prepro-SIFamide sv2 | 76 | F | 117 | 7e-27 | |
| Sulfakinin | TR6787|c0_g1_i1 | 1023 | Prepro-sulfakinin | 120 | F | 196 | 1e-50 |
| TRP | TR41108|c0_g1_i1 | 2211 | Prepro-TRP | 129 | F | 442 | 1e-124 |
| Trissin | TR50290|c0_g2_i1 | 943 | Prepro-trissin v1 | 187 | F | 58 | 7e-09 |
| TR50290|c0_g1_i1 | 940 | Prepro-trissin v2 | 186 | F | 56 | 2e-08 | |
Length in nucleotides.
Length in amino acids.
Peptide family abbreviations: ACP, adipokinetic-corazonin-like peptide; AST-A, allatostatin A; AST-B, allatostatin B; AST-C, allatostatin C; CAP2b, cardioacceleratory peptide 2b; CCAP, crustacean cardioactive peptide; CHH, crustacean hyperglycemic hormone; MIH, molt-inhibiting hormone; DH31, diuretic hormone 31; DH44, diuretic hormone 44; ETH, ecdysis-triggering hormone; EH, eclosion hormone; FLP, FMRFamide-like peptide; GPα2, glycoprotein hormone α2; GPβ5, glycoprotein hormone β5; ILP, insulin-like peptide; NPF, neuropeptide F; NPLP1, neuropeptide-like precursor 1; NPLP2, neuropeptide-like precursor 2; NPLP3, neuropeptide-like precursor 3; NPLP4, neuropeptide-like precursor 4; PDH, pigment dispersing hormone; PTTH, prothoracicotropic hormone; RPCH, red pigment concentrating hormone; sNPF, short neuropeptide F; TRP, tachykinin-related peptide.
Other abbreviations: C, carboxy-terminal partial protein; F, full-length protein; N, amino-terminal partial protein; sv, splice variant; v, variant.
Query proteins: ACP, Homarus americanus prepro-ACP (deduced from Accession No. GEBG01018127; Christie et al., 2015); AST-A, H. americanus prepro-AST-A (deduced from Accession No. GEBG01007827; Christie et al., 2015); AST-B, Procambarus clarkii prepro-allatostatin B (deduced from Accession No. GBEV01040422; Christie and Chi, 2015c); AST-C, H. americanus prepro-AST-C I (deduced from Accession No. GEBG01004053; Christie et al., 2015) and H. americanus prepro-AST-C II (deduced from Accession No. EY291152; Dickinson et al, 2009b); allatotropin, Tigriopus californicus prepro-allatotropin (deduced from Accession No. JW513825; Christie, 2014c); bursicon α, H. americanus pre-bursicon α (deduced from Accession No. GEBG01013055; Christie et al., 2015); bursicon β, H. americanus pre-bursicon β (deduced from Accession No. CN854188; Christie et al., 2010b); CAP2b, Nilaparvata lugens prepro-CAP2b splicing variant a (Accession No. BAO00940; Tanaka et al., 2014); CCHamide, H. americanus prepro-CCHamide I (deduced from Accession No. GEBG01016648; Christie et al., 2015) and H. americanus prepro-CCHamide II (deduced from Accession No. GEBG01015625; Christie et al., 2015); corazonin, H. americanus prepro-corazonin (deduced from Accession No. GEBG01047508; Christie et al., 2015); CCAP, H. americanus prepro-CCAP (deduced from Accession No. GEBG01001997; Christie et al., 2015); CHH, H. americanus prepro-CHH A (Accession No. P19806; de Kleijn et al., 1995); MIH, H. americanus pre-gonad-inhibiting hormone (Accession No. CAA60644; de Kleijn et al., 1994); DENamide, Daphnia pulex prepro-DENamide (Dircksen et al., 2011); DH31, H. americanus prepro-CLDH (Accession No. ACX46386; Christie et al., 2010c); DH44, H. americanus prepro-DH44 (deduced from Accession No. GEBG01010013; Christie et al., 2015); DXXRLamide, T. californicus prepro-DXXRLamide Ia (deduced from Accession No. JW528324; Christie, 2014c); ETH, D. pulex prepro-ETH (Dircksen et al., 2011); EH, pre-EH I (deduced from Accession No. GEBG01042722; Christie et al., 2015); elevenin, N. lugens prepro-elevenin (Accession No. BAO00952; Tanaka et al., 2014); FLP, H. americanus prepro-FLRFamide (deduced from Accession No. GEBG01004307; Christie et al., 2015); FXGGXamide, T. californicus prepro-FXGGXamide Ia (deduced from Accession No. JV193177; Christie, 2014c); GPα2, N. lugens pre-glycoprotein hormone α2 (Accession No. BAO00955; Tanaka et al., 2014); GPβ5, N. lugens pre-glycoprotein hormone β5 (Accession No. BAO00956; Tanaka et al., 2014); GSEFLamide, H. americanus prepro-GSEFLamide (deduced from Accession No. GEBG01035690; Christie et al., 2015); HIGSLYRamide, Carcinus maenas prepro-HIGSLYRamide (deduced from Accession No. DV111329; Christie et al., 2008b); ILP, H. americanus prepro-ILP (deduced from Accession No. GEBG01059205; Christie et al., 2015); intocin, H. americanus prepro-intocin (deduced from Accession No. GEBG01052869; Christie et al., 2015); leucokinin, H. americanus prepro-leucokinin (deduced from Accession No. GEBG01042414; Christie et al., 2015); myosuppressin, H. americanus prepro-myosuppressin (Accession No. ACX46385; Stevens et al., 2009); neuroparsin, H. americanus pre-neuroparsin (deduced from Accession No. GEBG01017677; Christie et al., 2015); NPF, H. americanus prepro-NPF (deduced from Accession No. GEBG01010211; Christie et al., 2015); NPLP1, N. lugens prepro-NPLP1 (Accession No. BAO00966; Tanaka et al., 2014); NPLP2, Drosophila melanogaster prepro-NPLP2, isoform A (Accession No. AAF49832; Adams et al., 2000); NPLP3, N. lugens prepro-NPLP3 (Accession No. BAO00967; Tanaka et al., 2014); NPLP4, N. lugens prepro-NPLP4 (Accession No. BAO00968; Tanaka et al., 2014); orcokinin, H. americanus prepro-orcokinin I (Accession No. ACB41787; Dickinson et al., 2009a); PDH, H. americanus prepro-PDH (deduced from Accession No. GEBG01005888; Christie et al., 2015); proctolin, H. americanus prepro-proctolin (deduced from Accession No. GEBG01005712; Christie et al., 2015); PTTH, N. lugens prepro-PTTH (Accession No. BAO00973; Tanaka et al., 2014); pyrokinin, H. americanus prepro-pyrokinin (deduced from Accession No. GEBG01039267; Christie et al., 2015); RPCH, Cherax quadricarinatus prepro-RPCH (Accession No. AAV80404; Martinez-Perez et al., 2007); RYamide, P. clarkii prepro-RYamide (deduced from Accession No. GBEV01010112; Christie and Chi, 2015c); sNPF, P. clarkii prepro-sNPF (deduced from Accession No. GBEV01004780; Christie and Chi, 2015c); SIFamide, H. americanus prepro-SIFamide (Accession No. ABV21807; Dickinson et al., 2008); sulfakinin, H. americanus prepro-sulfakinin (Accession No. ABQ95346; Dickinson et al., 2007); TRP, H. americanus prepro-TRP (Accession No. ACB41786; Christie et al., 2008a); trissin, D. melanogaster prepro-trissin (Accession No. AAF55203; Adams et al., 2000).