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Markers to identify cell lineages within in vitro cultures. The existence of differentiated, post-mitotic cell lineages was confirmed using biomarkers for identification: anti-CgA (enteroendocrine, panel A), anti-MUC2 (goblet, panel B) and anti-lysozyme (paneth, panel C) antibodies. All specific markers shown in red. Digital magnification of each image provided in the insert. Nuclei, blue. Scale bar 20 μm unless noted.
