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Functional Antibodies Used
| Cell Type | Antibody | Fixation | Dilution |
|---|---|---|---|
|
| |||
| Epithelial | EpCAM | WM | 1:250 |
| B-catenin | |||
| Proliferative cell | Mouse anti-human Ki67 | WM | 1:100 |
| PE | 1:250 | ||
| Intestinal epithelial stem cell | Rabbit anti-human SOX9 | WM | 1:500 |
| PE | 1:1000 | ||
| Enteroendocrine cell | Rabbit anti-porcine chromogranin A | WM | 1:500 |
| PE | 1:1000 | ||
| Goblet cell | Rabbit anti-human MUC2 | PE | 1:1000 |
| Paneth cell | Rabbit anti-human lysozyme | PE | 1:500 |
PE = Paraffin embedded; WM = Whole mount
