Table 2.
PCR Primer Sequences.
| Target | Forward (5′-3′) | Reverse (5′-3′) | Product Size (bp) |
|---|---|---|---|
| Active Stem Cell | |||
| SOX9 | AACAAGCCTCACGTCAAGCG | TCCGCCTCCTCCACGAAA | 176 |
| LGR5 | AGCCTGGTGGTTCTGCATCT | AACGCTTTCTCGGGGATCAG | 200 |
| Reserve Stem Cell | |||
| HOPX | AGTGGCGGCGGTCGGAA | ACAGTGGCACATACAACACT | 167 |
| LRIG | GGCTGACACAACTGGACCTC | TGGTGCCCGAAATGTCGTTA | 477 |
| Epithelial Cell | |||
| EPCAM | TGACCACAAACTGCTCTGTGA | AGCCCGTCGTTATTCTGGAT | 180 |
| Absorptive Enterocytes | |||
| SIM | GCAATACTGGGGGAAGCAGT | CAGATCCAGCAAAAGTTGACC | 137 |
| Goblet Cell | |||
| MUC2 | AGGACCTGAAGAAGTGCGTC | AGTTGAGAATCTTTCCTTCCTC | 165 |
| Enteroendocrine Cell | |||
| CGA | CTGCGAGGAGATGAACGGAT | AGAACCTCTGCGAGTTCGTC | 143 |
| Paneth cells | |||
| LYZ | CCTGGTCAGCCTAGAGGTCT | TGGCCAAACAGACCCAGTTT | 186 |
| Proliferating cells | |||
| PCNA | CATGGACTCGTCCCACGTC | CTTCAGCCCTTAGGGTAATG | 164 |
| Housekeeping Gene | |||
| B-ACTIN | CTGTGGCATCCACGAAACTA | GACAATGAGGCCAGAATGGA | 237 |