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. 2018 Jan 29;9:78. doi: 10.3389/fimmu.2018.00078

Figure 3.

Figure 3

Lack of core fucosylation suppressed the IgG class-switching by impaired CD4+ T cell activation. (A,B) Flow cytometry analysis of the proportion of CD4+ and CD8+ cells. SPLs were obtained from Fut8+/+ and Fut8−/− mice with or without OVA immunization. SPLs were filtered through nylon mesh, and resuspended. Numbers indicate the percentage of the total spleen cells within this quadrant, and 10,000 events were acquired for each analysis. Data are representative of three independent experiments. Data are shown as mean values ± SD (ns, not significant; *p < 0.05; **p < 0.01). The number of Fut8+/+CD4+ T and Fut8−/−CD4+ T cells were (1.29 ± 0.32) × 107 and (6.38 ± 0.89) × 106 of the SPLs, respectively (n = 5). (C) Comparison of the levels of serum immunoglobulin isotypes in Fut8+/+ and Fut8−/− mice after OVA immunization. The immunoglobulin isotypes are compared by mouse mAb isotyping reagents (Sigma). Comparable results were obtained in three independent experiments. Data are shown as mean values ± SD (*p < 0.05; **p < 0.01).