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. 2018 Jan 29;9:96. doi: 10.3389/fimmu.2018.00096

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Copyright © 2018 Zheng, Xu, Mao, Mou, Wu, Wang, Bu, Zhou, Luo, Lu, Liu, Yuan, Wang, Chen and Xiao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of interleukin-23 (IL-23) on intracellular reactive oxygen species (ROS) levels in Nthy-ori 3-1 cells. Nthy-ori 3-1 cells were incubated with the indicated concentrations of IL-23 for 3 h in the presence or absence of anti-IL-23 (5 µg/mL) pre-treatment, and the cellular ROS was probed for with DCFH-DA. (A) The DCF fluorescence was detected and analyzed immediately using immunofluorescence (200×; scale bars, 50 µm). (B,C) The intracellular ROS levels by flow cytometry analysis are presented by percentages of positive cells (% positive cells) and mean fluorescence intensity (MFI). The statistical results shown are representative of three replicates. Significant differences and P values are calculated with the one-way ANOVA. ns, not significant. **P < 0.01, ***P < 0.001.