Table 1. Composition of experimental diet.
Ingredients, g | ND | HPD |
---|---|---|
Corn starch | 150 | 150 |
Casein | 200 | 500 |
D,L-methionine | 3 | 3 |
Sucrose | 500 | 200 |
Cellulose powder | 50 | 50 |
Corn oil | 50 | 50 |
Mineral mixture* | 35 | 35 |
Vitamin mixture† | 10 | 10 |
Choline bitartrate | 2 | 2 |
tert-Butylhydroquinone | 0.01 | 0.01 |
Total, g | 1,000 | 1,000 |
ND, normal diet; HPD, high protein diet; AIN, American Institute of Nutrition.
*Composition of AIN-76A mineral mix (g/kg): calcium phosphate, dibasic 500; sodium chloride 74; potassium citrate, monohydrate 220; potassium sulfate 52; magnesium oxide 24; manganous carbonate (43%–48% Mn) 3.5; ferric citrate (16%–17% Fe) 6; zinc carbonate(70% ZnO) 1.6; cupric carbonate (53%–55% Cu) 0.3; potassium iodate 0.01; sodium selenite 0.01; chromium potassium sulfate 0.55; sucrose, finely powdered 118.03; †Composition of AIN-76A vitamin mix (g/kg): thiamin hydrochloride 0.6; riboflavin 0.6; pyridoxine hydrochloride 0.7; nicotinic acid 3.0; D-calcium pantothenate 1.6; folic acid 0.2; D-biotin 0.02; cyanocobalamine 0.001; cholecalciferol(400,000 IU/g) 0.25; manaquinone 0.005; ascorbic acid 0.2; sucrose, finely powdered 992.824.