Table 1.
Primer | Sequence | Annealing temperature (°C) |
---|---|---|
DAMD Markers | ||
HBV5 | GGTGTAGAGAGGGGT (15) | 50.6 |
HVR | CCTCCTCCCTCCT(13) | 44.0 |
INS | ACAGGGGTGGGG(12) | 42.0 |
URP9F | ATGTGTGCGATCAGTTGCTG (20) | 57.3 |
URP2R | CCCAGCAACTGATCGCACAC (20) | 61.4 |
URP25F | GATGTGTTCTTGGAGCCTGT (20) | 57.3 |
M13 | GAGGGTGGCGGCTCT (15) | 56.0 |
HBV3 | GGTGAAGCACAGGTG (15) | 50.6 |
ISSR markers | ||
N7 | GAGGAGGAGGC (11) | 38.0 |
N8 | CACACACACACAGT (14) | 42.0 |
N9 | ACACACACACACACACAG(18) | 53.7 |
N10 | ACACACACACACACACAA (18) | 51.4 |
N12 | ACACACACACACACACGA (18) | 53.7 |