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. 2018 Feb 2;8(2):109. doi: 10.1007/s13205-018-1108-3

Table 1.

List of DAMD and ISSR Primers and their annealing temperatures

Primer Sequence Annealing temperature (°C)
DAMD Markers
 HBV5 GGTGTAGAGAGGGGT (15) 50.6
 HVR CCTCCTCCCTCCT(13) 44.0
 INS ACAGGGGTGGGG(12) 42.0
 URP9F ATGTGTGCGATCAGTTGCTG (20) 57.3
 URP2R CCCAGCAACTGATCGCACAC (20) 61.4
 URP25F GATGTGTTCTTGGAGCCTGT (20) 57.3
  M13 GAGGGTGGCGGCTCT (15) 56.0
  HBV3 GGTGAAGCACAGGTG (15) 50.6
ISSR markers
 N7 GAGGAGGAGGC (11) 38.0
 N8 CACACACACACAGT (14) 42.0
 N9 ACACACACACACACACAG(18) 53.7
 N10 ACACACACACACACACAA (18) 51.4
 N12 ACACACACACACACACGA (18) 53.7