Table 3.
Inhibitors concentration (mM) | Relative activity (%) | ||
---|---|---|---|
1.0 | 2.5 | 5.0 | |
PMSF | 100.45 ± 3.98a | 122.86 ± 5.67a | 77.03 ± 5.92c |
EDTA | 100.87 ± 2.56a | 113.45 ± 8.25b | 75.68 ± 4.38c |
β-Mercaptoethanol | 87.61 ± 3.45b | 77.62 ± 6.39d | 68.73 ± 5.25d |
IAA | 100.95 ± 6.32a | 100.05 ± 5.63c | 93.25 ± 2.58b |
Control | 100.82 ± 4.28a | 100.24 ± 4.97c | 100.98 ± 3.67a |
Residual enzymatic activity was measured in 30 mM sodium carbonate/sodium bicarbonate buffer (pH 10.5) containing various enzyme inhibitors at 45 °C for 6 h. Values were expressed as mean ± SD (n = 3). Values that were not sharing a similar superscript within the same column were significantly different (P < 0.05) as determined by ANOVA