Table 8.

Periodic saccharification of liquefied corn starch using different concentrations of commercial enzyme

S. no.	Time (h)	Gluco-amylase ex. Rhizopus (in terms of reducing sugars mg/ml)
		20 U	40 U	60 U	80 U	100 U	500 U
1.	0.5	2.36 ± 0.05	2.61 ± 0.07	2.52 ± 0.02	2.64 ± 0.06	2.60 ± 0.07	3.2 ± 0.54
2.	1.0	2.52 ± 0.02	2.74 ± 0.05	3.10 ± 0.03	3.19 ± 0.06	4.10 ± 0.04	6.26 ± 0.05
3.	1.5	2.99 ± 0.03	2.97 ± 0.06	4.20 ± 0.04	4.28 ± 0.09	7.42 ± 0.07	10.62 ± 0.18
4.	2.0	3.31 ± 0.04	3.32 ± 0.05	6.76 ± 0.06	9.86 ± 0.04	11.89 ± 0.06	13.91 ± 0.07
5.	2.5	3.26 ± 0.02	2.98 ± 0.06	7.03 ± 0.04	9.79 ± 0.04	11.86 ± 0.07	14.46 ± 0.04
	CD@5%	0.112	0.177	0.126	0.189	0.193	0.421

Incubation temperature—60 °C