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. 2017 Dec 31;9(4):4301–4317. doi: 10.18632/oncotarget.23794

Figure 4. Niclosamide treatment induces apoptosis and suppresses CREB activation.

Figure 4

HL60 and KG1cells were treated with 1 μM niclosamide for 1d. A. Cells were fixed, and then stained with anti-cleaved PARP (cPARP) antibody and DAPI. Percentages of apoptotic cells are indicated. B. Fixed cells were stained with anti-phospho-CREB(p-CREB) (S133) and anti-phospho-NFkB p65 (S536) antibodies. Plots show the suppression of p-CREB in cells treated with niclosamide as compared with untreated control, but not phospho-NFkB p65. Percentages of p-CREB-negative population are presented as mean ± SEM (n = 3). C. Cells were stained with anti-phospho-STAT1 (Y701), anti-phospho-STAT3 (Y705), and anti-phospho-STAT5 (Y694) antibodies. HL60 cells: Top panels, KG1 cells: Bottom panels. Negative populations of each antibody were calculated. Values are graphed as mean ± SEM (n = 3). *, p < .05; **, p < .001.